The nucleotides involved in the P3 apt formation are underlined. Inserted in the 3’ UTR of the EGFP transcript between the XbaI (green) and BspEI (red) sites in pEGFP-N1-BspEI (Figure S1 and Appendix). (Excitation maximum = 488 nm emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. PEGFP-N1 Description pEGFP-N1 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells. Plasmid Type: Mammalian Expression Source/Vendor: Clontech Promotor: CMV
#Phy304 vector sequence in snapgene viewer free
This is a free resource for the scientific community that is compiled by Addgene. Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. pEGFP-N1 is between the immediate early promoter of CMV (P CMV IE) and the EGFP coding sequences.Sticky ends from different EcoO109I sites may not be compatible. PciI is inhibited by nonionic detergents. Sticky ends from different AflIII sites may not be compatible. For other reading frames, use pEGFP-N2 or pEGFP-N3. Vector for fusing EGFP to the C-terminus of a partner protein. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences. What is the excitation and emission maximum of EGFP? The MCS in pEGFP-N1 is between the immediate early promoter of CMV (P. Description: pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. If you wish to digest the vector with this enzyme, you will need to transform the vector into a dam– and make fresh DNA.
PEGFP-N1 is between the immediate early promoter of CMV and the EGFP coding sequences.